anti brd4 (Bethyl)
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Anti Brd4, supplied by Bethyl, used in various techniques. Bioz Stars score: 96/100, based on 513 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+brd4/BRD4+Antibody/pm41857039-390-0-8
Average 96 stars, based on 513 article reviews
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other:Article Title: Androgen receptor–mediated assisted loading of the glucocorticoid receptor modulates transcriptional responses in prostate cancer cells Article Snippet: Primary antibodies were as follows: anti-GR (Santa Cruz Biotechnology sc-1003, RRID:AB_631572), anti-GR (Cell Signaling Technology 12041, RRID:AB_2631286), anti-AR , anti-FOXA1 (Abcam ab23738, RRID:AB_2104842), anti-SMARCA4 (Abcam ab110641, RRID:AB_10861578), anti-BRD4 (Bethyl Laboratories A301-985A, RRID:AB_1576498), anti-GAPDH (Santa Cruz Biotechnology sc-25778, RRID: AB_10167668), antitubulin (Santa Cruz Biotechnology sc-5286, RRID:AB_628411), anti-BrdU (Abcam ab6326, RRID:AB_305426), and normal rabbit IgG (Millipore 12-370, RRID:AB_145841). Isolation:Article Title: Regulation of inflammatory responses by pH-dependent transcriptional condensates. Article Snippet: .. The isolated nuclei were washed (10 mM Tris-HCl pH 8, 1 mM EDTA, 0.5 mM EGTA, and 200 mM NaCl) and sheared in (0.1% sodium dodecyl sulfate (SDS), 1 mM EDTA, and 10 mM Tris-HCl pH 8) with the Covaris E229 sonicator for 10 min. After centrifugation, chromatin was immunoprecipitated overnight at 4 ◦ C with 1:100 anti-BRD9 (Bethyl, pAb #A700-153) or 5 μg Centrifugation:Article Title: Regulation of inflammatory responses by pH-dependent transcriptional condensates. Article Snippet: .. The isolated nuclei were washed (10 mM Tris-HCl pH 8, 1 mM EDTA, 0.5 mM EGTA, and 200 mM NaCl) and sheared in (0.1% sodium dodecyl sulfate (SDS), 1 mM EDTA, and 10 mM Tris-HCl pH 8) with the Covaris E229 sonicator for 10 min. After centrifugation, chromatin was immunoprecipitated overnight at 4 ◦ C with 1:100 anti-BRD9 (Bethyl, pAb #A700-153) or 5 μg Immunoprecipitation:Article Title: Regulation of inflammatory responses by pH-dependent transcriptional condensates. Article Snippet: .. The isolated nuclei were washed (10 mM Tris-HCl pH 8, 1 mM EDTA, 0.5 mM EGTA, and 200 mM NaCl) and sheared in (0.1% sodium dodecyl sulfate (SDS), 1 mM EDTA, and 10 mM Tris-HCl pH 8) with the Covaris E229 sonicator for 10 min. After centrifugation, chromatin was immunoprecipitated overnight at 4 ◦ C with 1:100 anti-BRD9 (Bethyl, pAb #A700-153) or 5 μg Article Title: BRD4 acts as a transcriptional repressor of RhoB to inhibit terminal erythropoiesis. Article Snippet: .. The following primary antibodies were used for immunoprecipitation or Western blotting: anti-IgG (Abcam, ab172730), Incubation:Article Title: Regulation of inflammatory responses by pH-dependent transcriptional condensates. Article Snippet: .. The isolated nuclei were washed (10 mM Tris-HCl pH 8, 1 mM EDTA, 0.5 mM EGTA, and 200 mM NaCl) and sheared in (0.1% sodium dodecyl sulfate (SDS), 1 mM EDTA, and 10 mM Tris-HCl pH 8) with the Covaris E229 sonicator for 10 min. After centrifugation, chromatin was immunoprecipitated overnight at 4 ◦ C with 1:100 anti-BRD9 (Bethyl, pAb #A700-153) or 5 μg Article Title: BRD4 acts as a transcriptional repressor of RhoB to inhibit terminal erythropoiesis. Article Snippet: 75,000 erythroblasts treated with DMSO or MS436 for 2 days were prepared for each sample, and technical triplicate were performed with Hyperactive Universal CUT&Tag Assay Kit for Illumina (Vazyme, TD903) following the manufacturer’s instructions. .. Briefly, erythroblasts were incubated with Concanavalin A beads and Chromatin Immunoprecipitation:Article Title: Regulation of inflammatory responses by pH-dependent transcriptional condensates. Article Snippet: .. The isolated nuclei were washed (10 mM Tris-HCl pH 8, 1 mM EDTA, 0.5 mM EGTA, and 200 mM NaCl) and sheared in (0.1% sodium dodecyl sulfate (SDS), 1 mM EDTA, and 10 mM Tris-HCl pH 8) with the Covaris E229 sonicator for 10 min. After centrifugation, chromatin was immunoprecipitated overnight at 4 ◦ C with 1:100 anti-BRD9 (Bethyl, pAb #A700-153) or 5 μg Article Title: Identification of super-enhancer-based biomarkers for predicting survival and immunotherapy efficacy in colorectal cancer Article Snippet: .. ChIP assays were conducted as reported in previous studies with the anti-H3K27ac (Abcam, ab177178, Cambridge, UK), anti-P300 (Abcam, ab275378, Cambridge, UK) and Western Blot:Article Title: BRD4 acts as a transcriptional repressor of RhoB to inhibit terminal erythropoiesis. Article Snippet: .. The following primary antibodies were used for immunoprecipitation or Western blotting: anti-IgG (Abcam, ab172730), |

